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• 1.Internship Report –Alkhidmat Diagnostic Center, Layyah Ayesha Saleem Reg# 21-CUVAS-0430 BS Medical Laboratory Technology 2021-2025
• 2.Introduction • Duration: 5thFeb 2025 – 5th May 2025 • Supervisor: Dr. Afsan Amir Sumra • Key Focus Areas: • Clinical Pathology (Urine/Semen analysis) • Biochemistry (LFTs, RFTs, Electrolytes) • Hematology (CBC, PT/APTT, ESR) • Serology (HBsAg, HCV, HIV, Malaria testing)
• 3.Phlebotomy & SampleCollection • Vacutainer Types & Uses: • Red: Chemistry tests (LFTs, RFTs) • Lavender: CBC (EDTA anticoagulant) • Light Blue: Coagulation tests (PT/APTT) • Key Steps: Patient ID, vein puncture,  proper mixing, labeling.
• 4.Hematology Lab Highlights •CBC (SYSMEX XS-1000i): Parameters: – Hb, RBCs, WBCs, Platelets. Normal Values: – E.g., Hb (Male: 13.9–16.9 g/dL). • Coagulation Tests: PT (11–15 sec),APTT(26–32 sec) INR for warfarin monitoring. • ESR (Westergren Method): • Measures inflammation.
• 5.Biochemistry Lab Highlights •Routine Chemistry Indiko Analyzer: – Tests: Glucose, – Uric Acid, – LFTs (ALT, AST), – RFTs (Creatinine). • Electrolytes (Elite Ultra): Na , K , Cl . ⁺ ⁺ ⁻ • Special Chemistry: Lifotonic ECL-8000: – Thyroid Profile (T3, T4, TSH), – Troponin I.
• 6.Blood Bank &Urine/Semen Analysis Blood Grouping: Forward/reverse typing (e.g., A+, B+). Cross matching: Ensures donor compatibility. Urine Analysis: • Physical (color, turbidity) • Chemical (dipstick), • Microscopic (WBCs, RBCs). Semen Analysis: • Motility, sperm count.
• 7.Serology Lab Highlights •Rapid Tests (Manual Kits): – HBsAg, HCV, HIV: Positive = 2 lines (C + P). – Malaria Parasite: Detects Plasmodium antigens. – ASO Test: Strep infection (agglutination = positive).
• 8.Key Learnings &Skills • Operated analyzers (SYSMEX, Indiko, Lifotonic). • Performed manual tests (ESR, blood grouping, serology). • Learned QC protocols and safety measures. • Improved teamwork and patient interaction skills.
• 9.Conclusion • Gained hands-onexperience in diagnostic procedures. • Bridged theoretical knowledge with practical application.
• 10.Thank You!